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CNAA / Theses / 2012 / July /

Natural Variability and Biosynthetic Activity of Actinomycetes Streptomyces massasporeus


Author: Bratuhina Antonina
Degree:doctor of biology
Speciality: 03.00.07 - Microbiology
Year:2012
Scientific adviser: Svetlana Burţeva
doctor habilitat, professor, Institute of Microbiology and Biotechnology of the ASM
Scientific consultant: Vladimir Şeptiţchi
doctor habilitat, associate professor (docent), Institute of Physiology and Sanacreatology of the ASM
Institution: Institute of Microbiology and Biotechnology of the ASM
Scientific council: DH 09-03.00.07
Institute of Microbiology and Biotechnology of the ASM

Status

The thesis was presented on the 11 July, 2012
Approved by NCAA on the 9 October, 2012

Abstract

Adobe PDF document – 0.91 Mb / in romanian
Adobe PDF document – 0.81 Mb / in russian

Keywords

streptomycetes, changeability, amino acids, lipids, fatty acids, antimicrobial activity, EMR of extremely high frequencies of low-intensity, stress.

Summary

The thesis consists of the introduction, 4 chapters, general conclusions and recommendations, the bibliography of 210 references, 10 annexes, 118 pages of the text, 44 pictures, 14 tables. The results of the research are published in 19 scientific works.

Key words are: streptomycetes, changeability, amino acids, lipids, fatty acids, antimicrobial activity, EMR of extremely high frequencies of low-intensity, stress. Domain of study: 03.00.07 – Microbiology. Aim of the work: investigation of natural variability and biosynthetic activity of the cultures of actinomycetes Streptomyces massasporeus after a long-term storage and exposure to low-intensity of electromagnetic radiation of extremely high frequencies, as well as the prospects of using physiologically active substances synthesized by these cultures to increase animals’ organism resistance to stress factors. Tasks: analysis of natural variability and biosynthetic activity of the cultures of actinomycetes S. massasporeus after a long-term storage; study of the influence of EMR of extremely high frequencies of low-intensity upon variability and biosynthetic activity of actinomycetes S. massasporeus; elaboration of cultivation conditions to increase biosynthetic activity of S. massasporeus CNMN-Ac-06 strain; study of the exposure of biologically active components of the biomass and culture liquid of S. massasporeus CNMN-Ac-06 strain to some morphologic, functional, reproductive indices and behavioral characteristics of white rats in chronic stress condition. Scientific problem: determination of variability of S. massasporeus strains, a producer of biologically active substance; possibility of controlling their synthesis and the prospects of their usage. Scientific novelty and originality: a homologous series of intraspecific variability of the population of pigmented actinomycetes S. massasporeus was revealed for the first time; differences of morpho-cultural features and biosynthetic activity of strains depending on the type of their storage were determined for the first time. The perspective of the usage of EMR of extremely high frequencies of low-intensity for decrease of pigmented actinomycetes S. massasporeus heterogeneity and increase of their biosynthetic activity were elicited for the first time. The strain S. massasporeus CNMN-Ac-06 correlation of lipid saturation and lipoxygenase activity depending on phosphorus concentration in medium were revealed. The fact that biologically active components of the biomass and culture liquid of S. massasporeus CNMN-Ac-06 strain forward the normalization of the actively transported food substances absorption process in the small intestine as well as the increase of the body weight and facilitation of the defensive conditioned reflex training in the chronic stress condition was revealed for the first time. Theoretical significance consists in perfecting actinomycetes of the genus Streptomyces taxonomy, demonstrating the possibility of controlling of biosynthetic processes, and streptomyces heterogeneity by means of EMR of extremely high frequencies of low-intensity and by changing cultivation conditions. The peculiarities of action of the substances synthesized by S. massasporeus CNMN-Ac-06 to some morphologic, functional, reproductive indices and behavioral characteristics of white rats in chronic stress condition were studied, which can be the basis for new biopreparations elaboration. These biopreparations are intended for intensity of growth increase, fertility, and macroorganisme resistance to bad conditions. Practical importance: agarous nutritional media for storage in laboratory conditions of S. massasporeus strains providing a good growth and maximal homogeneity of population are recommended. Nutritional media for deep cultivation increasing biosynthetic activity of streptomycetes are proposed. EMR of extremely high frequencies of low-intensity parameters providing homogeneity of the population of pigmented actinomycetes S. massasporeus and stimulating their biosynthetic activity were revealed. The obtained results made possible to use the biomass and culture liquid of S. massasporeus CNMN-Ac-06 strain for stress disorder of homoitherms prophylaxis and correction. Implementation of scientific results. Nutritional media for keeping in laboratory conditions of actinomycetes S. massasporeus strains and EMR of extremely high frequencies of low-intensity parameters providing minimal variability, good growth, maintenance of biosynthetic activity were proposed. The basic results of the investigation are implemented into work of the National Collection of Nonpathogenic Microorganisms of the IMB of the ASM and in the educational process at the Medical Department of the Shevchenko USN.