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CNAA / Theses / 2010 / July /

Biotechnology of obtaining the preparations based on astaxanthin from Haematococcus pluvialis Flotow CNM-AV-05


Author: Miscu Vera
Degree:doctor of biology
Speciality: 03.00.23 - Biotechnology
Year:2010
Scientific adviser: Valeriu Rudic
doctor habilitat, professor, Institute of Microbiology and Biotechnology of the ASM
Institution: Institute of Microbiology and Biotechnology of the ASM
Scientific council:

Status

The thesis was presented on the 20 July, 2010
Approved by NCAA on the 6 October, 2010

Abstract

Adobe PDF document – 1.54 Mb / in romanian

Keywords

astaxanthin, Haematococcus pluvialis, biotechnologies, preparations based on oil, antioxidative activity

Summary

Dissertation contains Introduction, 4 Chapters, Main Conclusions and Practical Reccomendations, List of literature, consisting from 237 titles, 10 appendix, 120 pages of main text, 12 tables and 46 pictures. The results of the work were published in 13 papers.

Goal of the work: development of new technologies for obtaining of the preparations based on astaxanthin from Haematococcus pluvialis Flotow CNM-AV-05.

Tasks: adaptation and development of extraction methods, quantification and stabilization of astaxanthin from H. pluvialis biomass; Development of H. pluvialis cultivation methods, maximising the astaxanthin output; Development of the obtaining methods for the preparations based on astaxanthin from H. pluvialis; Estimation of antioxidative activity for the preparations based on astaxanthin and study of their mechanisms.

Scientific novelty. Efficiency of extraction from raw, dried, lyophilized and frozen H. pluvialis biomass was demonstrated using ethanol or plant oils; as well as the ability to increase the astaxanthing production using the coordination compounds of Fe(III), Zn(II) and Co(II,III). First the method of reduction the phosphorus molibdenium reagent was used to estimate the antioxidative activity of astaxanthin.

Theoretical importance: New data were obtained on the ability to manage the biosynthetic processes in H. pluvialis to increase the astaxanthin output using coordination compounds of Zn(II), Fe(III), Co(II) and Co(III); mechanisms of antioxidative and antiradical properties of the preparations based on astaxanthin; ability of oil preparation of astaxanthin to inhibit NO development and oxidation of low density lipoproteins.

Applied importance. Complex technologies for the obtaining of preparations based on astaxanthin from H. pluvialis, developed in this work, give the opportunities for optimization and further improvement of efficacy in the production of astaxanthin preparations.

Application of scientific results. The technologies for the obtaining of lipidic preparations on the basis of astaxanthine of algal origine have been implemented on the „Ficotehfarm” LTD