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Genetic and molecular variability of Salvia sclarea L. genotypes


Author: Martea Rodica
Degree:doctor of biology
Speciality: 03.00.15 - Genetics
Year:2017
Scientific adviser: Maria Duca
doctor habilitat, professor, Moldova State University
Institution: Institute of Genetics, Physiology and Plant Protection of the ASM

Status

The thesis was presented on the 8 December, 2016
Approved by NCAA on the 16 February, 2017

Abstract

Adobe PDF document – 1.46 Mb / in romanian

Thesis

CZU 653.743 : 631.526 (478)

Adobe PDF document 1.89 Mb / in romanian
142 pages

Keywords

gene expression, genetic and molecular polymorphism, phytochemical variability, Salvia sclarea L.

Summary

The thesis includes introduction, 6 chapters, conclusions and recommendations, bibliography consisting of 295 sources, 77 pages of main text, 33 tables, and 55 figures. The results of the research are reflected in 15 publications.

Field of investigation: Plant genetics.

The purpose of the researchis the establishment of genetic, molecular and biochemical variability of S. sclarea genotypes from Republic of Moldova, the association of results at DNARNA- protein-metabolites levels, in order to describe processes related to the potential of secondary metabolism and to identify the possibility of directed selection of genotypes with economically valuable traits.

Objectives of the thesis have focused on the establishment of S. sclarea variability by application of bioinformatics approaches, the estimation of the clary sage variability by demonstration the genetic polimorfism, evaluation of LPPS and HPPR genes expression level , phytochemical analysis of sclareol and polyphenolic compounds, and correlation of results obtained at studied levels of organization.

Scientific novelty and originality of the research consist in establishing for the first time of the high biochemical and molecular-genetic variability and the specific particularities, that reveal the high ameliorative capacity of local S. sclarea germplasm, in determining the expression level of the LPPS gene, responsible for the synthesis of lambda-13-en-8ol diphosphate synthase which participate in the formation of sclareol, as well as HPPR gene responsible for synthesis of the hydroxyphenylpyruvate reductase which participate in the formation of the rosmarinic acid.

Important scientific problem solved is scientific validity of the ameliorative potential of S. sclarea genotypes by biochemical and molecular data correlation, which led to the identification of three hybrids (H3, H4 and H8) with a higher biosynthetic potential for major compounds as sclareol and polyphenol acids, expressed as rosmarinic acid, that allows to improve process efficiency and creation of highly competitive hybrids.

The theoretical significance is supported by the integrative methodology of analyzing the biochemical and molecular-genetic data and the LPPS and HPPR genes expression correlation with the amount of biologically active compounds (sclareol and polyphenols), which contribute to improve the knowledge about genetic-molecular mechanisms of secondary metabolites synthesis and offer new possibilities of predicting productive forms on the base of the complex of indicators.

The applicative value of the work is related to the identification of polymorphic spectrum and the inheritance of RAPD amplicons, quantification of sclareol and polyphenolic content in the clary sage genotypes, to the highlighting the hybrids with increased biosynthetic capacity, as well as elaboration and implementation in biological research by the UDaCoT tool and Med Plant data base resources.

Implementation of scientific results. The RAPD primers and specific primer developed for LPPS and HPPR genes are used in the study of S. sclarea species within the Centre for Functional Genetics and are recommended for subsequent molecular-genetic studies. The scientific results can be used as reference support in amelioration of this species; obtained results represent scientific and didactic material in teaching courses of Genetics, Bioinformatics and Biochemistry. The hybrids H3, H4 and H8, which showed higher biosynthetic capacity, are recommended for the assessment and implementation in creation of varieties of hybrid origin.