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CNAA / Theses / 2019 / May /

The biosynthetic properties of Streptomyces fradiae CNMN-Ac-11 and the physiological effects of biomass on the organism of homoioterm animals (rats)


Author: Bereziuc Iulia
Degree:doctor of biology
Speciality: 03.00.07 - Microbiology
Year:2019
Scientific adviser: Svetlana Burţeva
doctor habilitat, professor, Institute of Microbiology and Biotechnology of the ASM
Scientific consultant: Vladimir Şeptiţchi
doctor habilitat, associate professor (docent)
Institution: Institute of Microbiology and Biotechnology of the ASM

Status

The thesis was presented on the 30 May, 2019
Approved by NCAA on the 9 July, 2019

Abstract

Adobe PDF document – 0.81 Mb / in romanian
Adobe PDF document – 1.01 Mb / in russian

Thesis

CZU 579.873.71+[574.36:599.323.4](043.3)

Adobe PDF document 3.07 Mb / in russian
144 pages

Keywords

streptomycetes, amino acids, lipids, carbohydrates, antimicrobial activity, nutrient medium, physiological effects, white rats.

Summary

The thesis consists of an introduction, four chapters, conclusions and recommendations, bibliography list with 211 references. It comprises 119 pages of main content, 31 figures, 12 tables and 6 annexes. The results were published in 13 scientific papers. Key words: streptomycetes, amino acids, lipids, carbohydrates, antimicrobial activity, nutrient medium, physiological effects, white rats.

Field of study: 163.04 – Microbiology.

Research goal consists in research of the cultural properties and biosynthetic activity of the strain Streptomyces fradiae CNMN-Ac-11 and substantiation of the possibility of using it as a biomass producer, affecting the physiological parameters of the organism of homoioterm animals (white rats) under normal physiological conditions and under the action of chronic stress.

Objectives: the study of the cultural properties of the strain Streptomyces fradiae CNMNAc-11; determination of the ability to accumulate biomass and synthesis of basic lipid fractions, amino acid and carbohydrate composition during the cultivation of the strain Streptomyces fradiae CNMN-Ac-11 on different media; study of the effect of substances of cyanobacterial origin on the ability of the strain Streptomyces fradiae CNMN-Ac-11 to actively increase biomass with altered qualitative composition; study of the biomass of the strain Streptomyces fradiae CNMN-Ac-11 on morphological, functional, reproductive indicators, as well as on the quantitative characteristics of individual representatives of the intestinal microflora, homoioterm animals (white rats) under normal conditions and under chronic stress.

Scientific novelty and originality. The data on the qualitative and quantitative composition of the physiologically active lipid fractions of the strain Streptomyces fradiae CNMN-Ac-11 were obtained. For the first time, the effect of using substances of cyanobacterial origin as an additional component to the main nutrient medium was revealed. The biomass of the strain Streptomyces fradiae CNMN-Ac-11 was found to help normalize the number of the intestinal microflora microorganisms, the process of glucose absorption in the small gut, increase body weight gain, facilitate the development of conditioned reflexes.

The main scientific problem solved in the study consists in the elucidation of the biosynthetic activity of the strain Streptomyces fradiae CNMN-Ac-11 - a producer of biologically active substances, which allows to manage the processes of increasing the amount of biomass, amino acids and lipid fractions, determining the prospect of using it with the aim of improving the functional and reproductive parameters of white rats under chronic stress.

Theoretical value. The possibility of increasing the amount of physiologically active lipid fractions using substances of cyanobacterial origin as stimulants of their synthesis in actinomycetes of the genus Streptomyces has been scientifically substantiated. The effect of the biomass of the strain Streptomyces fradiae CNMN-Ac-11 on the functional and reproductive characteristics of white rats was studied.

Applicative value. The proposed strain Streptomyces fradiae CNMN-Ac-11, characterized by high biosynthetic activity; new nutrient media options have been proposed to increase the amount of Streptomyces fradiae CNMN-Ac-11 biomass; Experimental data have outlined the prospects for the development of drugs based on the biomass of the strain Streptomyces fradiae CNMN-Ac-11.

Implementation of scientific results. The main results of the research are implemented in the educational process of the Faculty of Natural Sciences of the Shevchenko USN, as well as in the work of the National Collection of Non-pathogenic Microorganisms IMB.