Molecular profile of the stroma in benign and malignant proliferations of the prostate
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StatusThe thesis was presented on the 7 September, 2022Approved by NCAA on the 25 November, 2022 Abstract – 1.44 Mb / in romanianThesisCZU 616.65-006-018(043.2)
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Keywords
prostate carcinoma, prostate adenoma, reactive stroma, extracellular matrix, α-SMA+cells, CD68+ macrophages, S100+ cells, mast cells, angiogenesis, androgen receptorsSummary
Thesis structure:introduction, 5 chapters, general conclusions and recommendations, bibliography of 236 titles, 119 pages of the main text, 77 figures, 23 tables, 3 annexes. The obtained results have been published in 12 scientific papers.
The aim of the research: To study the stromal components of the prostate by evaluating the role of stromal factors in the tumorogenesis, in order to improve the histological diagnosis.
The research objectives: The histological and immunohistochemical studying of stromal changes (cellular and extracellular components) in benign and malignant proliferative lesions of the prostate; studying of the blood vessles network, vascular density and vessel types in relation to the severity of hyperplastic prostate lesions; evaluation of the intensity of androgen receptor expression by stromal and glandular cells.
The scientific novelty and originality: For the first time, a complex and comparative study of stromal changes of the two prostatic hyperplastic lesions has been performed. The usage of three mast cell antibodies allowed their immunophenotyping, as well as the establishment of an unic mast cell immunophenotype in the peritumoral areas of the prostate adenocarcinomas. It has been established that AR stromal expression in the prostate carcinoma decreases with increasing tumor histologycal degree, that can be interpreted as a natural selection of negative AR stromal cells versus positive AR cells. It has been established that stromal dendritic cells can be considered a prognostic elements.
The scientific solved problem: The reactive stroma already appears in the benign prostate proliferative lesions; macrophages, mast cells and dendritic cells reprogram each other, contributing to the secretion of biologically active substances with a regulatory role in the processes of proliferation and angiogenesis; decreased stromal dendritic cell count in the prostate carcinoma is an unfavorable prognostic factor.
The theoretical significance and applicative value of the research: Analyzing the evolution of quantitative and qualitative changes of cell populations, and extracellular matrix components, it was possible to supplement the existing knowledge with new data about the involvement of stroma in the prostate proliferative processes, as well as to confirm the usefulness of histological and immunohistochemical methods in the diagnosis and assessment of the severity of hyperplastic lesions of the prostate gland.
The implementation of scientific results: The obained results of this research were implemented
in the teaching and scientific processes of the Department of Histology, Cytology and Embryology
Oficial Reviewers
- Viorel Nacu
doctor habilitat, professor, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova - CĂRUNTU Irina-Draga
doctor în medicină, profesor universitar,, UMF “Grigore T. Popa”, Iași, România
Council's Members
- Fulga Veaceslav, president
doctor habilitat, associate professor (docent) - Valeriu David, member
doctor, associate professor (docent) - Mihail Ştefaneţ, member
doctor habilitat, professor, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova - Ion Dumbraveanu, member
doctor habilitat, associate professor (docent), Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova - CEAUȘU Raluca Amalia,
doctor în medicină, conferențiar universitar, România
Theses
