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The elaboration and assessment of the rapid methods in the laboratory diagnosis of urinary tract infections


Author: Bălan Greta
Degree:doctor of medicine
Speciality: 03.00.07 - Microbiology
Year:2009
Scientific adviser: Iurie Roşcin
doctor habilitat, professor
Scientific consultant: Adrian Tanase
doctor habilitat, professor, State University of Medicine and Pharmacy "Nicolae Testemitanu"
Institution: State University of Medicine and Pharmacy "Nicolae Testemitanu"
Scientific council: DH 09-03.00.07
Institute of Microbiology and Biotechnology of the ASM

Status

The thesis was presented on the 25 December, 2008
Approved by NCAA on the 26 February, 2009

Abstract

Adobe PDF document0.58 Mb / in romanian

Thesis

CZU [616.61-022.1+616.98]-078

Adobe PDF document 16.00 Mb / in romanian
131 pages


Keywords

urinary tract infections, rapid methods, nutritive medium, laboratory diagnosis

Summary

The paper represents a cycle of scientific-applicative researches on development and improvement of diagnosis methods, cultivation medium and microreagents for rapid diagnosis of the urinary tract infections.

Experimentally, in laboratory conditions, there were determined a selective acceleration factors for rapid growth and multiplication of ethiologic agents in the urinary tract infections, on the basis of which there were developed and improved the following:


As a rezult, there was developed a scheme for rapid diagnosis of urinary tract infections, which permit the cultivation, indication and determination of susceptibility of the ethiological agents of the UTI till 24 hours (from the moment of collection and insemination of the pathological material).

There were carried out 340 specimen, from them 217 (63,8%) – positive. In 203 (93,6%) cases there was observed one isolated, but in 14 (6,4%) cases – 2 isolated. From the positive results in 164 (75,6%) cases enterobacterial microorganisms there were isolated; 38 (17,5%) cases – staphylococci; 12 (5,5%) cases – bacteria P.aeruginosa and 3 (1,4%) cases – levuriform fungus from Candida spp.

The developed methods and microreagents are rapid, economical, sensible, specific and simple in using. They are accesible in microbiological laboratories of all levels in the curative-prophylactic network of public health services.