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CNAA / Theses / 2009 / July /

Safety and pharmacological aspects of entomological drug IMUPURINE


Author: Pogonea Ina
Degree:doctor of medicine
Speciality: 14.00.25 - Pharmacology, Clinical Pharmacology
Year:2009
Scientific adviser: Victor Ghicavîi
doctor habilitat, professor, State University of Medicine and Pharmacy "Nicolae Testemitanu"
Institution:
Scientific council:

Status

The thesis was presented on the 28 July, 2009
Approved by NCAA on the 1 October, 2009

Abstract

Adobe PDF document0.29 Mb / in romanian

Keywords

imupurin, pupae, immunomodulatory, immunotrope, toxicity, immunodeficiency, phagocytosis, non specific resistance, Lepidoptera

Summary

The work is dedicated to the study of safety and pharmacological proprieties of imupurine, an entomologic drug obtained from Lepidoptera pupae. In researching the acute toxicity of the medicine, the conclusion drawn is that imupurine doesn’t have systemic toxicity in enteral administration, and in that parenteral the average lethal dose (DL50) is within a large range (160 + 16,3 mg/kg). Long-term administration of the entomologic drug (30 days) proved innocuous, with a positive dynamic in the body weight and improvement in the functions of the liver. Under these circumstances, redistribution in the leucocyte formula, with the growth in the percentage of lymphocytes was witnessed.

Experimental studies demonstrate that imupurin exhibits important immunotropic proprieties. Thus, as experimentally established, the drug in doses of 100 and 1000 mcg for a mouse significantly increases the varietal (unspecific) resistance of animals, as testified by the survival of 70 – 80 % of mice infected with lethal doses of golden cluster seed (staphylococcus aureus).

Simultaneously, imupurine significantly intensifies phagocytic processes as revealed by the growth in the number of neutrophils and phagocytant macrophages, which proves the intensification of functional activity of neutrophils and macrophages.

The entomological drug exerted significant influence on the humoral and cellular immunity. In that way, imupurine contributed to the normalization of lymphocytes B and T. Simultaneously, considerable changes in the population of T lymphocytes take place, with the increase of T h, responsible for the formation of interleucines, with subsequent activation of B lymphocytes, their blast transformation and production of immunoglobulins.

In simulating hepatic affections with paracetamol, the entomological drug exhibited hepatoprotecting proprieties both in the pretreatment and in the medical treatment. Imupurine contributes to the diminution of the cytolytic syndrome (diminution of the AlAT and AsAT levels), correction of metabolic and morphologic deregulations.

Imupurine manifests immunotropic and hepatoprotecting proprieties through its components – the content of essential and non- essential amino acids, polypeptides, lipoproteic complexes and antioxidants, which confers the drug a complex immunomodulating and hepatocyte protection mechanism.