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Morpho-physiologic and genetic aspects of host-parasite interaction (Helianthus annuus L. - Orobanche cumana Wallr.)


Author: Rotarenco Victoria
Degree:doctor of biology
Speciality: 03.00.12 - Plant physiology
Year:2010
Scientific adviser: Maria Duca
doctor habilitat, professor, Moldova State University
Institution: Moldova State University
Scientific council: DH 10-03.00.12-27.03.08
Institute of Genetics, Physiology and Plant Protection of the ASM

Status

The thesis was presented on the 30 September, 2010
Approved by NCAA on the 4 November, 2010

Abstract

Adobe PDF document – 1.11 Mb / in romanian

Keywords

Helianthus annuus L., Orobanche cumana Wallr., host-parasite, resistance, molecular markers

Summary

The thesis consist of introduction, five chapters, conclusions and recommendations, 336 sources of bibliography, a total of volume 128 pages, 20 tables, 41 figures. The obtained results were published in 12 scientific papers.

Direction of investigation: 03.00.12 - Plant physiology.

Research goals. The purpose of this work was to study the morpho-physiological and genetic aspects of host-parasite interaction, based on sunflower and broomrape.

Objectives: estimation of the degree of infection in H. annuus genotypes under artificial infection with O. cumana; identification and molecular analysis of Or5 gene in 53 homo- and heterozygous genotypes of sunflower; revealing some nucleotide and protein sequences which are implicated in defense response to O. cumana.

Scientific novelty and originality. Complex studies, morphologic, physiologic and genetic, have been carried out in different sunflower genotypes infected with broomrape.

Theoretical significance. The obtained data bring a new contribution in explication of physiological and genetic mechanism of resistance to broomrape, at pre-haustorial and post-haustorial level.

Applicative value of the work. Seven sunflower genotypes resistant to broomrape (FS9, FS12, FS17, FS21, FS26, FS27 and one androsteril line ASC1) have been identified, which can be used in further research and breeding. Implementation of scientific results.

The results of the study represent a scientific and methodological support for teaching courses of Plant physiology, Genetics, Ecophysiology and Plant adaptability, and also as a theoretical tool in the creation of sunflower lines and hybrids resistant to broomrape.