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Identification and expression of some sunflower (Helianthus annuus L.) genes (Rf)

Author: Midoni Andrei
Degree:doctor of biology
Speciality: 03.00.15 - Genetics
Scientific adviser: Maria Duca
doctor habilitat, professor, Moldova State University
Institution: Moldova State University
Scientific council: DH 10-03.00.15-27.03.08
Institute of Genetics and Plant Physiology of the ASM


The thesis was presented on the 30 December, 2010
Approved by NCAA on the 24 February, 2011


Adobe PDF document1.37 Mb / in romanian


sunflower, fertility restorer genes, CMS-Rf system, bioinformatics, hybridologic analysis


The thesis consists of introduction, 6 chapters, conclusions, bibliography of 285 sources, a total of 119 pages, 23 tables and 53 pictures. The results are published in 10 papers on the topic of research.

Research goal: molecular study of male sterility / fertility mechanisms, identification of restorer of fertility genes in different genotypes grown in Republic of Moldova, while research of inheritance and fertility restoration mechanisms in sunflower.

Objectives: bioinformatics’ analysis of different CMS-Rf systems; genetic polymorphism (RAPD) in CMS, Rf and F1 correlated with morphological features; number determination and type interaction of Rf genes by hybridologic analysis; identification and status evaluation of Rf genes using CAPS-PCR analysis; presence / absence estimation of maize marker linked with Rf3 gene (CMS-S) in different sunflower genotypes; analysis of some aspects associated with Rf genes expression.

Scientific novelty: similar features were observed between CMS-PET 1 sunflower and CMS-S maize systems, at the same time was identified molecular markers linked to Rf3 gene (maize) in Rf and F1 sunflower genotypes; was determined cytoplasmatical (Rf) and membranar location of CMS proteins in plants; was modeled polynucleotide-phosphorilase enzyme involved in sunflower restoration processes by polyadenylation of CMS transcripts witch is then digested by RNases; was distinguished the presence and Rf1 gene status (rf1rf1, Rf1Rf1 and Rf1rf1) in 9 maternal, 14 paternal and 16 hybrid genotypes;

Theoretical significance: was developed a hypothetical model of CMS-Rf components interaction in plants.

Applied value: in new created hybrids Drofa CMS x LC Raus Rf and Drofa CMS x VIR 681 Rf was estimated the high auto compatibility degree – important agronomic character.

Implementation of scientific results: the obtained results are successfully applied in genetics, selection (NOVOSEM SRL thesis annex 1), in bioinformatics, plant physiology. Based on hybridologic, CAPS-PCR and morphologic analysis we recommend the homozygous lines (Rf1Rf1): LC 4 Rf, LC 39 Rf, LC 41 Rf, LC 43 Rf, LC Cium Rf and LC Raus Rf to be used for creation of highly productive hybrids.