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Biotechnology of cultivation of the Aspergillus niger 33-19 CNMN FD 02A strain, amylases producer


Author: Stratan Maria
Degree:doctor of biology
Speciality: 03.00.23 - Biotechnology
Year:2012
Scientific adviser: Alexandra Deseatnic-Ciloci
doctor, associate professor (docent), Institute of Microbiology and Biotechnology of the ASM
Scientific consultant: Valeriu Rudic
doctor habilitat, professor, Institute of Microbiology and Biotechnology of the ASM
Institution: Institute of Microbiology and Biotechnology of the ASM
Scientific council: DH 09-03.00.23
Institute of Microbiology and Biotechnology of the ASM

Status

The thesis was presented on the 15 December, 2011
Approved by NCAA on the 16 February, 2012

Abstract

Adobe PDF document0.51 Mb / in romanian
Adobe PDF document0.51 Mb / in romanian

Keywords

micelial fungi, amylolytic enzymes, coordinative compounds of metals, millimetric waves with low intensity

Summary

Introduction, four chapters, conclusions, bibliography of 215 references, 12 anexes, 122 pages of the base text, 32 figures, 29 tables. The obtained results are published in 27 scientific papers.

The aim: elaboration of advanced technologies of amylolytic enzymes obtaining by directed synthesis on the submerged cultivation of the Aspergillus niger 33-19 CNMN FD 02A fungal strain.

Objects: morphological stability of the Aspergillus niger 33-19 CNMN FD 02A as biotechnological object evaluation; nutritive medium and cultivation conditions optimization; metallocomplexes efficiency and electromagnetic radiations in millimetric diapason determination and their use conditions as enhancing factors of amylases biosynthesis by the studied strain elaboration.

Scientific novelty and originality: a new source of amylases was developed – Aspergillus niger 33-19 CNMN FD 02A fungal strain. Coordination compounds of Co(III) with fluorinated anions and electromagnetic radiation in millimeter diapason under specified conditions stimulates the enzymatic synthesis of Aspergillus niger 33-19 CNMN FD 02A strain – amylases producer (MD 3654,MD 3943, MD 3945). Biostimulating action of the influence factors is achieved by enhancing mechanism of growth and multiplication of microbial cell.

The originality of the thesis is application of directed synthesis with the use of 3d elements and electromagnetic radiation in millimetric diapason as regulator factors in fungal enzymatic synthesis.

Scientific problem solved in the thesis consists in argument of ways to increase the synthesis of amylase from fungi mycelium with the application of physical and chemical factors of influence - coordination compounds of 3d elements and electromagnetic radiation in the millimeter range.

Theoretic significance: the obtained results show the perspective of coordinative compounds and electromagnetic radiation in millimetric diapasons regulation strategies of microbial biosynthesis and denote important contributions to the elucidation of influence mechanisms.

Application value: two high yield technologies of amylolytic enzymes obtaining through directed synthesis in submerged cultivation of Aspergillus niger 33-19 CNMN FD 02A strain with application of coordinative compounds and electromagnetic radiation in millimetric diapason as regulator factors were elaborated.

Scientific results implementation: the obtained enzyme amylolytic preparations were tested in the baking process (SA „Franzeluta”, Act 12-03/27, 05.11.2010) and as a compound of a complex enzyme preparation with application in zootechnics (Î.S. „MOLDSUINHIBRID”, Act of 20.06.2009).