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Productivity and biochemical composition of cyanobacterium Spirulina platensis CNM-CB-02 of the cultivation in the present of coordination compounds of copper


Author: Batîr Ludmila
Degree:doctor of biology
Speciality: 03.00.07 - Microbiology
Year:2013
Scientific adviser: Valeriu Rudic
doctor habilitat, professor, Institute of Microbiology and Biotechnology of the ASM
Scientific consultant: Aurelian Gulea
doctor habilitat, professor, Moldova State University
Institution: Institute of Microbiology and Biotechnology of the ASM
Scientific council: DH 09-03.00.07
Institute of Microbiology and Biotechnology of the ASM

Status

The thesis was presented on the 26 December, 2012
Approved by NCAA on the 14 June, 2013

Abstract

Adobe PDF document0.71 Mb / in romanian

Keywords

Spirulina platensis CNM-CB-02, bioactive principles, coordination compounds of Cu(II), antimicrobial coppercomponents preparations

Summary

The dissertation contains introduction, 4 chapters, main conclusion and practical recommendations, bibliography of 221 titles, 11 appendixes, 119 pages of main text, 21 tables and 22 pictures. The results of the work were published in 27 papers.

Field of research: 03.00.07 — Microbiology.

Goal of the work: determine whether the use of new coordination compounds of copper to adjust productivity, copper content and bioactive principles in Spirulina platensis biomass.

Thesis objectives: evaluation changes of productivity and biochemical composition of spirulina biomass and establish spirulina capacity of copper accumulation and distribution in the different fractions extracted from spirulina biomass; comparative study of morphological indices of spirulina and spectrum of polypeptides from protein fraction; elaboration of procedures to obtain spirulina biomass containing copper and bioactive principles.

Scientific novelty and originality: demonstrated the effectiveness of spirulina cultivation in the presence of coordination compounds of Cu(II) and obtaining biomass with high content of bioactive substances and organically bound copper. Were established qualitative and quantitative changes in protein spectrum and evaluated morphological and biochemical features of spirulina. It was determined copper content and distribution in fractions, establishing the possibility of obtaining coppercomponents preparations with antimicrobial activity.

Research problem: identification spirulina’s ability to fix Cu from coordination compounds and determining the morphological and biochemical results, which would serve to develop technologies for obtaining copper-enriched organic material.

Theoretical importance: we obtained new scientific results on the reactions of spirulina cultivation in the presence of coordination compounds of Cu(II), metal accumulation in different fractions of spirulina biomass and obtain coppercomponents preparations.

Applied importance: to maintain the biological activity of copper fixed in organic components of spirulina has been determined. Two procedures for obtaining biomass coppercomponents and two procedures for obtaining biomass with high content of phycobiliproteins were developed.

Applications of scientific results: procedures to obtain spirulina biomass containing copper and phycobiliproteins have been implemented on the „Ficotehfarm‖ LTD.