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Molecular and genetic aspects of heterosis in sunflower (Helianthus annuus L.)


Author: Ana Căpăţână
Degree:doctor of biology
Speciality: 03.00.15 - Genetics
Year:2006
Scientific adviser: Maria Duca
doctor habilitat, professor, Moldova State University
Institution:
Scientific council:

Status

The thesis was presented on the 23 March, 2006
Approved by NCAA on the 29 June, 2006

Abstract

Adobe PDF document1.88 Mb / in romanian

Thesis

CZU 577.21: 633.854.78

Adobe PDF document 4.60 Mb / in romanian
121 pages


Keywords

sunflower (Helianthus annuus L), heterosis, genetic polymorphism

Summary

For the first time have been made some complex studies of four hybrids and its parental lines, which permitted to appreciate the genetical polymorphism of studied genotypes and to reveal new genetical and molecular aspects of heterosis.

It was found out that mitotic activity was more intensive at heterozigote genotypes in comparison with homozigote lines. The relationships between productivity characters of this crop and its association with heterosis were revealed.

The determination of quantitative and qualitative peculiarities of total and fractionated proteins allowed to put in evidence the genetic polymorphism at homo- and heterozigote sunflower’s genotypes and to obtain new arguments and proofs in support of existent hypotheses and theories of heterosis, especially dominant and codominant inheritance of protein and DNA molecular components. The level of polymorphism correlates with the manifestation of different agronomical indexes.

Cluster analysis established that according to total proteins the studied genotypes formed two clusters: homo- and heterozigotes, whereas according to globulins the genotypes were grouped in dependence of their families. The clusters obtained as the result of the analysis of RAPD products of amplification, grouped genotypes in accordance to their yield.