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CNAA / Theses / 2006 / May /

Studding of Separation and Stabilisation of Anthocyanic Colorants from Aronia Melanocarpa

Author: Alexei Baerle
Degree:doctor of chemistry
Speciality: 02.00.04 - Physical chemistry
Scientific adviser: Vasile Guţanu
doctor habilitat, professor, Moldova State University
Scientific council:


The thesis was presented on the 12 May, 2006
Approved by NCAA on the 28 September, 2006


Adobe PDF document0.41 Mb / in romanian


CZU 544.3 : 66.067 : 547.97

Adobe PDF document 0.93 Mb / in romanian
118 pages


anthocyanines, mathematic modelling method, physical and chemical factors, quantitative analysis, ion-exchangers, sinegrism of desorbtion, cristalin preparates, anti radical activity


This work deals with studding of influence of different factors over stability and transformations of the anthocyanines in the complex extracts, in the modelling systems, and in the solid phase of the adsorbent. The mathematic modelling method and optimisation was applied.

Was determined, that the nature of organic solvents and the solid substances are very important for anthocyanin’s stability. This influence is complicate, including the solubility of molecular oxygen, water activity and generation of free radicals in the liquid phase.

Was elaborated a new express-method for quantitative determination of glycoside/aglicon ratio, which characterised the degree of hydrolysis and further degradation of natural anthocyanines. This method is based on the selective extraction of aglicon, with the solution of 3-metil-1-butanol : n-heptane (85 : 15).

The mechanism of transformation of the anthocyanines in the presence of sulphocationit includes depolymerisation and rapid acid hydrolysis, catalysed with the functional groups of the ion-exchanger.

The bright acid-ethanol synergism of desorbtion of the anthocyanines from the sulphocationit is discovered. Cause of these unusual phenomena is the blockage of associated anthocyanines in the three-dimensional structure of ion-exchanger and their decomposition under the influence of ethanol molecules.

Two national patent (Republic of Moldova) for the new procedures of obtaining of extra-pure anthocyanines were registered.

The antiradicalic and antioxidant activity of these samples is substantial. The antiradicalic activity of cyanidin is better, that of the ascorbic acid one. In the natural extracts of Aronia the basic antiradicalic agents are cyanidin-glycosides.