StatusThe thesis was presented on the 11 May, 2006
Approved by NCAA on the 29 June, 2006
Abstract– 0.16 Mb / in romanian
1.45 Mb /
The researches have established that the activity of all studied lysosomic enzymes, excepting the cathepsin G, in neutrophils' suspensions irradiated by helium plasma, has decreased. It shows lymphocytes that have higher sensibility for this physical factor than neutrophils, because in lymphocytes the activity of cathepsins D and G and acid phosphatase have decreased, but the activity of cathepsin LH, β-galactosidase, N-acetil-β-D-glucosaminidase and alkaline phosphatase have decreased. The component of plasma - electromagnetic waves, UV specter, has a different effect than that of influence of whole helium plasma, on the enzymatic activity in these cells. In the same time with the changes in the activity of lysosomic enzymes, produced by cold helim plasma, the level of HPL in neutrophils and lymphocytes is increasing, depending on the duration of irradiation. The activity of the enzymes from the antioxydative system in neutrophils and lymphocytes, when influenced by this physical factor, is depending on the dose of irradiation; at low doses the cold helium plasma has an antioxydative activity, at high doses the activity of AOS enzymes is down, which is proved by low activity of superoxyddismutase (SOD), catalase (CAT) and glutathionic cycle's enzymes, excepting glutathion-s-transferase. This enzyme, involved in detoxifying of POL products, maintains its activity also during extreme conditions created by this physical factor. The changes, induced by the helium plasma radiation influencing on the neutrophils and lymphocytes, are described by correlation between the parameters of the functional statement of lysosomic profile and the parameters of prooxydative and antioxydative system in these cells. In lymphocytes, the correlation between these parameters is negative. In neutrophils, the cold helium plasma radiation has established negative correlation between the parameters of lysosomic enzymes activity, AOS parameters and the HPL level. As exception, the correlation is positive between the main parameters of the activity of prooxydative and antioxydative system and the activity of cathepsin D and elastase. The estimation of phagocytar activity of neutrophils shows a dose-dependent activation induced by cold helium plasma radiation. This activation is produced by both mechanisms of intracellular destruction: O2 dependent and O2 independent, proved by the increasing of phagocytar number (PhN) and phagocytar index (PhI) in all studied groups.