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Diagnostics of the Apple stem pitting virus in the certification system of the fruit planting material


Author: Prodaniuc Leonid
Degree:doctor of biology
Speciality: 06.01.11 - Plant Protection
Year:2016
Scientific adviser: Leonid Voloşciuc
doctor habilitat, professor, Institute for Plant Protection and Ecological Agriculture of the ASM
Scientific consultant: Constantin Dadu
doctor habilitat, professor, Practical Scientific Institute of Horticulture and Food Technology
Institution: Practical Scientific Institute of Horticulture and Food Technology

Status

The thesis was presented on the 28 September, 2016
Approved by NCAA on the 15 November, 2016

Abstract

Adobe PDF document – 2.22 Mb / in romanian
Adobe PDF document – 1.62 Mb / in russian

Thesis

CZU 634.1[632.35+632.38](478)

Adobe PDF document 5.20 Mb / in russian
130 pages

Keywords

virus, pitting, apple, optimization, methods, fruit seed species, testing,certification

Summary

Thesis consists of the introduction, four chapters, the main conclusions and recommendations, bibliography of 164 sources.

The work is presented on 100 pages of the main text; it contains 12 tables, 25 figures and 16 supplements. The results within the dissertation were published in 11 scientific articles.

Aim of research: To improve and optimize the diagnostics methods of the Apple stem pitting virus and apply them in the system of fruit seed planting material certification according to the European certification standards.

Objectives: Isolation and identification of the virulent strains of the apple stem pitting virus. Determination of the reaction on the apple stem pitting virus transmission to the herbal indicators. Optimization of the apple stem pitting virus purification method. Elaboration of the diagnostic antiserum for the apple stem pitting virus. Improving and optimization the apple stem pitting virus diagnostic methods. Implementation of the optimized diagnostic methods in the system of certification of fruit seed planting material.

Scientific originality. For the first time in the Republic of Moldova the highly specified antiserum, which is capable to determine ASPV using ELISA method, is prepared. The PCR method for ASPV diagnostics is optimized. The method «Immunocapture», based on the obtained diagnostic antibodies is carried out. The calendar dates for the ASPV determining are elaborated.

The important solved scientific problem consists in the scientific substantiation of methodological approaches of the elaboration and optimization of the ASPV diagnostics methods in the system of production of the virus-free planting material. The results of the study of the obtained antiserums specificity, the implementing of the optimized methods and the determination of the calendar terms of the testing for the ASPV presence allowed to elaborate, improve and apply the optimized methods in the system of the fruit seed species virus-free planting material certification.

The theoretical significance consists in the elaborating and improving of the reliable methods of the apple stem pitting virus diagnostics, testing and re-testing of the fruit planting material, which serve as a theoretical basis for rapid testing methods of the other two latent apple viruses. Improvement and optimization of diagnostic methods will allow solving a number of theoretical and practical problems of transferring fruit nursery on the international certification standards.

The practical significance of the work. The obtained experimental data allow recommending the utilization of the optimized methods for getting the virus-free clones in order to transfer the Republic’s nursery on the virus free basis and the international certification standards.

Implementation of scientific results: the obtained results are implemented in the Laboratory of Virology (PSIHFP) and are widely used in the virus-free clones obtaining and in the farmers SRL „Surinmih”, SRL „GelnacrAgro”, GȚ „Melnic Ioana Feodor”, GȚ „Cebotari Feodor Mihail”, GȚ „Ghilinschii Dumitru” and in the fruit seed species nurseries.