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Antimicrobial effects of some new chemical compounds from local sources

Author: Lozan-Tirsu Carolina
Degree:doctor of medicine
Speciality: 03.00.07 - Microbiology
Scientific adviser: Valeriu Rudic
doctor habilitat, professor, Institute of Microbiology and Biotechnology of the ASM
Scientific consultant: Aurelian Gulea
doctor habilitat, professor, Moldova State University
Institution: Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova


The thesis was presented on the 19 January, 2017
Approved by NCAA on the 23 March, 2017


Adobe PDF document0.80 Mb / in romanian


CZU 615.281 : 616.095

Adobe PDF document 7.87 Mb / in romanian
167 pages


Antimicrobial activity, coordinative compounds, copper, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Shigella sonnei, Salmonella enterica


The thesis contains an introduction, 4 chapters, conclusions and recommendations, bibliography list with 240 references, consisting of 127 pages of the main text, 21 figures, 26 tables and 3 annexes. The results of work were published in 26 scientific papers.

Field of study: 313.02 – Medical microbiology and virology.

Aim: Evaluation of antimicrobial properties of new chemical compounds and their mechanisms of action on pathogens.

Objectives: To identify the antimicrobial action of the copper (II) coordination compounds with various ligands on the reference strains of pathogens; to determine the antimicrobial activity of aromatic propenone with thioamide/isothiocyanate on the reference strains of pathogens; to compare the antimicrobial activity of new chemical compounds with standard antiseptic activity; to define the specificity of new compounds antimicrobial activity on coproculture isolates; to establish the mode of action of new compounds on pathogenic microorganisms.

Scientific novelty of research: For the first time new chemical compounds with different structures were tested for antimicrobial activity and was compared their activity with antibacterial activity of standard antiseptics. The influence of new compounds on the antioxidant status of pathogen culture was estimated.

Important scientific problem, solved in this research work, is identification of antimicrobial properties of new local chemical compounds and determination of the peculiarities of their actions.

Theoretical signification: New scientific data regarding the effects of some new compounds on the reference strains and clinical pathogen isolates have been accumulated. New data regarding the changes in the antioxidant status of pathogens under the influence of new chemical compounds have been collected. The stable correlation between the parameters of the antioxidant activity and the level of lipid peroxidation have been found, which proves the feasibility of this parameters as indicators in the process of testing of new compounds.

Practical value: New chemical compounds with antimicrobial activity against Staphylococcus aureus and Bacillus cereus, have been identified and proposed for continuous testing in vivo. The original method to determine the biomass of pathogens has been proposed.

Scientific results implementation. The study results are implemented as didactic material at the Department of Microbiology, Virology and Immunology. The method for bacterial biomass determination was implemented in the National Collection of Nonpathogenic Microorganisms of IMB (Act no.74, 28 May 2015).