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CNAA / Theses / 2017 / May /

Immunobiochemical changes upon the administration of sulfated polysaccharides from spirulina in normal and pathological condition

Author: Mihalciuc Olga
Degree:doctor of medicine
Speciality: 14.00.36 - Laboratorial Diagnostics; Immunology and Alergology
Scientific adviser: Valentin Gudumac
doctor habilitat, professor, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova
Scientific consultant: Larisa Procopişin
doctor, associate professor (docent), Public Medical Sanitary Intitution Institute of Phthisiopneumology "Chiril Draganiuc"
Institution: Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova


The thesis was presented on the 19 May, 2017
Approved by NCAA on the 31 May, 2017


Adobe PDF document0.89 Mb / in romanian


CZU 612.396.11+582.26+612.017;577.1

Adobe PDF document 3.45 Mb / in romanian
172 pages


sulfated polysaccharides from spirulina, toxic hepatopathy, experimental immunodeficiency, asthma, imunobiochemical indices, cytokines, glutathione system


Field of study: 314.03 - Diagnostic Laboratory. The aims of the study: to evaluate the particularities and significance of the modifications of immunobiochemical (IMB) indices induced by the remedies containing sulphated polysaccharide from Spirulina (SPS) in the normal and in pathological conditions with implication of the immune system and toxic nature diseases. Objectives of the study: to study the action of SPS on the blood parameters, IMB indices and metabolic processes in the serum, liver, spleen and bone marrow in the normal conditions, toxic hepatopathy (TH) and experimental immunodeficiency (ID), in vitro elucidation of the particularity of the IMB processes in the blood of asthmatic patients and the influence of the SPS. Research methodology: a fundamental research on in vivo laboratory animal and in vitro on biological samples collected from healthy individuals and patients with asthma were effectuated. Over 50 assays micro methods developed on base of existing methods were used. The IBM indices were studied in blood, liver and spleen tissues, and bone marrow under physiological conditions, TH, ID, asthma and after treatment with SPS and remedies containing SPS. The TH was induced by CCl4 administration and ID was modeled by cyclophosphamide. The results were processed using a statistical software "StatsDirect" and "Statistics 6.0" and presented in tabular and graphical form. The novelty and the scientific originality: For the first time, the multiplane complex research study was conducted, which allowed us to obtain new data on the IMB indices modifications in the blood, liver, spleen and bone marrow in the pathological conditions with implication of the immune system and toxic nature deseases. The beneficial action of the administration of SPS in toxic hepatopathy, experimental ID, and atopic patients with various severity was demonstrated. Theoretical significance: Elucidation of IMB mechanisms, underlying the action of the SPS on the organism in the normal and pathological conditions, expand the theoretical knowledge about the biological properties of natural compounds, offering new opportunities to explore perspective objects to obtain new efficient therapeutic remedies. The practical value of the work: Obtained data can be used to develop new schemes for early diagnosis of immune and biochemical disorders that occur in immune pathologies and toxic nature, and for monitoring of the efficiency of applied treatment. The pharmacological properties of the SPS can be tested in other diseases, the therapeutic applications may be extended. Implementation of scientific results: In the teaching process and scientific work of the Department of Laboratory Medicine of The State University of Medicine and Pharmacy Nicolae Testemitsanu, and in the clinical diagnostic laboratory of Institute of Cardiology and Republican Center of External Quality Control.