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Biotechnology of the obtaining of the immunomodulator BioR(SpI) and the study of its action on the immunological reactivity and natural resistance “in vitro” of lung tubercular patients”

Author: Victoria Darii
Degree:doctor of biology
Speciality: 03.00.23 - Biotechnology
Scientific advisers: Serghei Ghindă
doctor habilitat, professor, Public Medical Sanitary Intitution Institute of Phthisiopneumology "Chiril Draganiuc"
Valeriu Rudic
doctor habilitat, professor, Institute of Microbiology and Biotechnology of the ASM
Scientific council:


The thesis was presented on the 27 October, 2006
Approved by NCAA on the 21 December, 2006


Adobe PDF document0.55 Mb / in romanian


CZU 573.6:577.112.38:615.37

Adobe PDF document 1.20 Mb / in romanian
132 pages


ficobiotechnology, spirulina, biomass, immunoactive amino acids, immunomodulater BioRSpI, immunosupression, immunostimulation, , the immune reactivity, natural resistance, tuberculosis


The work is based on the results of the investigations dedicated to the elaboration of the technologies of obtaining of the new immunomodulator classes with an increased containing of immunoactive amino acids in the basis of biomass of the Spirulina platensis CNM-CB-02 (CYANOPHYTA) and the research of its action on the immunological reactivity and natural resistance.

Thus, there was elaborated the original technology of spirulina cultivation with a predictive containing of immunoactive amino acids in the basis of which the new preparation BioRSpI whith immunomodulatory action was obtained.

The containing of immunoactive amino acids is higher in the preparation BioRSpI than in preparation BioR, for example the containing of the glycine, valine, alanine, glutamic acid is 1,6-2,3 times higher, serine, threonine, asparagine is 3,2-4,3 times higher, tryptophan, arginine, cysteine is 6,5-8 times higher.

There was examined 99 patients of different age, both male and female sex with diverse forms of pulmonary tuberculosis and 50 healthy persons (control group), where “in vitro” means that preparation BioRspi has both stimulatory and suppressive action on the functional activity of T lymphocytes and the containing of glucose, consummated from the cultivation medium, the containing of T, T-TFR, T-TFS lymphocytes, B and 0 lymphocytes, on the containing of neutrophiles, the functional activity of neutrophiles.

The normalizing optimal dose of the preparation, needed for the regulation of the changed parameters of the immunological reactivity and natural resistance, is individual and situated at different limits, starting with the undiluted preparation up to the dilution of 1:512.

The action of the preparation BioRSpI is dependent of the initial parameters of the immunological reactivity and natural resistance. Thus, the preparation BioRSpI has stimulatory action at the patients with the low initial parameters (subnorma), suppressive action at the patients with increased parameters and the preparation has no action, when parameters are situated in norma limits.

As a result, such a mechanism of action on the changed parameters of immunological reactivity is characteristic for immunomodulators from the adaptogens group, that permits to classificate the preparation BioRSpI to the group of immunomodulators of vegetal origin (adaptogens).