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Biochemical markers of hypertensive retinopathy


Author: Pavlovschi Ecaterina
Degree:doctor of medicine
Speciality: 03.00.04 - Biochemistry
Year:2022
Scientific advisers: Olga Tagadiuc
doctor habilitat, professor
Eugeniu Bendelic
doctor habilitat, professor, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova
Institution: Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova

Status

The thesis was presented on the 4 May, 2022 at the meeting of the Scientific Council and now it is under consideration of the National Council.

Abstract

Adobe PDF document0.50 Mb / in romanian
Adobe PDF document0.53 Mb / in english

Thesis

CZU 617.735:616.12-008.331.1-078:577.1(043.2)

Adobe PDF document 2.76 Mb / in romanian
118 pages


Keywords

hypertensive retinopathy, oxidative stress, reactive oxygen species, antioxidant system, hypoxia, renin-angiotensin system

Summary

The aim of the research was to study the role of oxidative stress, antioxidant system (AOS) and renin-angiotensin system in the pathogenesis of hypertensive retinopathy and the identification of laboratory markers for the diagnosis and monitoring of the pathology.

Research objectives:
1. The study of changes in OS and antioxidant system indices in serum and tear of patients with primary hypertension with varying degrees of HR.
2. The evaluation of serum and tear RAS markers in patients with primary hypertension with varying degrees of HR.
3. The correlations identification of biochemical changes with the severity of HR.
4. The establishment of biochemical markers for early diagnosis and prognosis of HR evolution.

Scientific research methodology. An analytical, observational study was performed on a representative sample of patients with primary HTN with HR without specific antihypertensive treatment, divided into study subgroups according to the severity of HR, respecting all scientific requirements and ethical principles of institutional, national and international research. In order to achieve the objectives of the thesis, tears and blood serum were investigated to assess the metabolic indices of OS, antioxidant and RAS systems and ischemia. The Research Ethics Committee positive decision was obtained on 08.02.2018 (no. 35/34).

The novelty and scientific originality of the obtained results. The research complemented the current knowledge on the pathogenesis of HR, analyzing biochemical changes and their reflection in blood and tears. Markers of OS, antioxidant protection, ischemic damage and RAS were measured in both fluids, with the appreciation of the presence of tear–blood serum correlation of the evaluated indices. It has been shown that the eye is structurally autonomous, with specific defensive systems, which later allows it to withstand the effects of OS. The idea of the lack of a single reliable index, relevant for all HR stages was suggested, the appreciation of clinical manifestations tending to remain a priority.

Approval of scientific results. The research results were presented, discussed and approved at several national and international scientific forums: Annual Scientific Conference of Institute of Emergency Medicine Specialists “News and controversies in medical-surgical emergency management”, Chisinau, Moldova, November 10, 2017; 7th International Medical Congress for Students and Young Doctors MedEspera, “Nicolae Testemitanu” SUMPh, Chisinau, Moldova, May 3-5, 2018; Annual scientific conference of young specialists within IMSP IMU "Performances and perspectives in medical and surgical emergencies", Chisinau, Moldova, May 18, 2018; Annual Scientific Conference of Institute of Emergency Medicine "News and controversies in the management of medical and surgical emergencies", Chisinau, Moldova, December 7, 2018; VII Bukovinian International Medical Congress, BIMCO 2020, Bukovinian State Medical University, Chernivtsi, Ukraine, April 7-10, 2020. Congress dedicated to the 75th anniversary of the founding of “Nicolae Testemitanu” SUMPh, Chisinau, Moldova, October 20-23, 2020; XXIst International Scientific and Practical Conference International Trends in Science and Technology, Warsaw, Poland, January 31, 2020; 7th Lublin International Medical Congress, LIMC 2020, Medical University of Lublin, Lublin, Poland, 26-28 November 2020; VIIIth Bukovinian International Medical Congress, BIMCO 2021, Bukovinian State Medical University, Chernivtsi, Ukraine, April 6-9, 2021; International Scientific Conference on Medicine 2021, University of Latvia, Riga, Latvia, 23-24 April 2021; EURETINA 2021 Virtual, 9-12 September 2021. Keywords: hypertensive retinopathy, oxidative stress, reactive oxygen species, antioxidant system, hypoxia, renin-angiotensin system.