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CNAA / Theses / 2022 / /

Molecular profile of the stroma in benign and malignant proliferations of the prostate

Author: GLOBA Tatiana
Degree:doctor of medicine
Speciality: 14.00.15 - Pathological Anatomy
Scientific advisers: Lilian Şaptefraţi
doctor habilitat, professor, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova
RAICA Marius
doctor în medicină, profesor universitar, UMF “Victor Babeș”, Timișoara, România
Institution: Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova


The thesis was presented on the 7 September, 2022
Approved by NCAA on the 25 November, 2022


Adobe PDF document1.44 Mb / in romanian


CZU 616.65-006-018(043.2)

Adobe PDF document 4.72 Mb / in romanian
166 pages


prostate carcinoma, prostate adenoma, reactive stroma, extracellular matrix, α-SMA+cells, CD68+ macrophages, S100+ cells, mast cells, angiogenesis, androgen receptors


Thesis structure:introduction, 5 chapters, general conclusions and recommendations, bibliography of 236 titles, 119 pages of the main text, 77 figures, 23 tables, 3 annexes. The obtained results have been published in 12 scientific papers.

The aim of the research: To study the stromal components of the prostate by evaluating the role of stromal factors in the tumorogenesis, in order to improve the histological diagnosis.

The research objectives: The histological and immunohistochemical studying of stromal changes (cellular and extracellular components) in benign and malignant proliferative lesions of the prostate; studying of the blood vessles network, vascular density and vessel types in relation to the severity of hyperplastic prostate lesions; evaluation of the intensity of androgen receptor expression by stromal and glandular cells.

The scientific novelty and originality: For the first time, a complex and comparative study of stromal changes of the two prostatic hyperplastic lesions has been performed. The usage of three mast cell antibodies allowed their immunophenotyping, as well as the establishment of an unic mast cell immunophenotype in the peritumoral areas of the prostate adenocarcinomas. It has been established that AR stromal expression in the prostate carcinoma decreases with increasing tumor histologycal degree, that can be interpreted as a natural selection of negative AR stromal cells versus positive AR cells. It has been established that stromal dendritic cells can be considered a prognostic elements.

The scientific solved problem: The reactive stroma already appears in the benign prostate proliferative lesions; macrophages, mast cells and dendritic cells reprogram each other, contributing to the secretion of biologically active substances with a regulatory role in the processes of proliferation and angiogenesis; decreased stromal dendritic cell count in the prostate carcinoma is an unfavorable prognostic factor.

The theoretical significance and applicative value of the research: Analyzing the evolution of quantitative and qualitative changes of cell populations, and extracellular matrix components, it was possible to supplement the existing knowledge with new data about the involvement of stroma in the prostate proliferative processes, as well as to confirm the usefulness of histological and immunohistochemical methods in the diagnosis and assessment of the severity of hyperplastic lesions of the prostate gland.

The implementation of scientific results: The obained results of this research were implemented in the teaching and scientific processes of the Department of Histology, Cytology and Embryology