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Biotehnology of spirulina cultivation and obtaining of biomass fortified in iron and other valuable substances

Author: Liliana Zosim
Degree:doctor of biology
Speciality: 03.00.23 - Biotechnology
Scientific adviser: Valeriu Rudic
doctor habilitat, professor, Institute of Microbiology and Biotechnology of the ASM
Scientific council:


The thesis was presented on the 10 September, 2007
Approved by NCAA on the 18 October, 2007


Adobe PDF document0.36 Mb / in romanian


phycobiotechnology, Spirulina platensis, bioactive compounds, organic iron, coordinative compounds of Fe(III), biopreparations, anemia


The work constitutes a review focused on the studies of a possibility of application of 2 type of Fe(III) coordinative compounds: trinuclear Fe(III) clusters with amino acids and heterotrinuclear Fe(III) as regulators of spirulina growing, development and elaboration of technologies for biomass enriched in iron and bioactive compounds obtaining.

The results of the investigations have demonstrated the prospective of coordinative Fe(III) compounds application as regulators of biochemical composition of cyanobacteria Spirulina platensis(Nordst.) Geitl. CNM-CB-02 biomass. Due the using of mathematical planning methods of experiments were elaborated the procedures of phycobiliproteines and polysaccharides synthesis by Spirulina platensis and obtaining of biomass with high content of proteins (peptides), amino acids and iron – a source of antianemic products.

The iron distribution in diverse fractions from spirulina biomass have been estimated and its including in macro- and micro molecular organic compounds, such as peptides, proteins, amino acids and polysaccharides have been demonstrated. The essential part from iron total quantity is included in the fractions of amino acids, peptides and proteins, where were distributed: 881 ([Fe3O-Ala]), 900 ([Fe3O-Gly]) and 914 mg% Fe from biomass ([Fe2MgO]).

The technological scheme of new antianemic nutraceutic products „SpiruFierM” obtaining and new biopreparations, including antianemic remedies “BioR Fe”, have been elaborated on the basis of the proposed procedures. The investigations „in vitro” relived the fact, that the preparation “BioR Fe” is an adaptogen and manifests a complex action over the deregulated parameters of immunologic activity and natural resistance of organism and could be used as immunomodulator. The investigations „in vivo” have demonstrated its antianemic effect, expressed throw normalization of the main sanguine index in anemia caused by nutritional and toxic factors, due its properties to stimulate erythropoiesis, thrombocytopoiesis, leucopoiesis and concomitant recovering of plasmatic iron and zinc level at the experimental animals.

The results of investigations promote to solve the problems connecting to elaboration of the biotechnologies for bioactive compounds obtaining from spirulina fortified biomass and to use efficiently the biosynthesis products in different areas of medicine.