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«Развитие и микроклональное размножение вида Lycium barbarum L.(годжи)


Автор: Tabăra Maria
Степень:доктор биологических наук
Специальность: 03.00.05 - Ботаника
Год:2020
Научный руководитель: Nina Ciorchina
доктор, доцент, Ботанический сад (институт) АНМ
Институт:

Статус

Диссертация была зашищена 5 ноября 2020 в ученом совете и находится на рассмотрении в Национальном Совете.

Автореферат

Adobe PDF document1.06 Mb / на румынском
Adobe PDF document0.93 Mb / на английском

Диссертация

CZU 582.951.4(478) (043.2)

Adobe PDF document 9.99 Mb / на румынском
156 страниц


Ключевые слова

Lycium barbarum L., in vitro, микроклонирование/микрочеренкование, каллусогенез, ризогенез, акклиматизация, сорта годжи, фенология, анатомия, биохимия

Аннотация

Structure of the thesis. The thesis includes introduction, four chapters, general conclusions and practical recommendations, bibliography from 321 sources, total volume of 156 pages, 18 tables, 39 figures and 10 annexes. The obtained results are published in 21 scientific papers.

Field of investigation: 164.01 – Botany The purpose of the research is to develop in vitro multiplication technology of Lycium barbarum L. and comparative morphological and biochemical evaluation of biochemical compounds of the spontaneous species and varieties grown under the pedoclimatic conditions of the Republic of Moldova.

Objectives of the thesis: *identification of goji varieties and highlighting the appropriate explants for initiating in vitro cultures *selection of nutrients suitable for each stage of microcloning *analysis of plant development biology obtained, in vitro, ex vitro and experimental conditions; *anatomical study and comparative biochemical evaluation of the content of flavonosides, tannins, and ascorbic acid in cultivated goji plants and spontaneous flora; *elaboration of micropropagation technologies and description of the protocol for obtaining a high multiplication coefficient for goji varieties and extension of the genus of fruit shrubs within GBNI (initiation of the collection).

Scientific novelty and originality. For the first time in the Republic of Moldova, goji plants have been propagated through efficient micropropagation techniques and methods for the production of robust, uncontaminated and uniform propagating material. This complex study is the first of its kind in our country, its importance being given by the need for biological characterization of shrubs in the experimental group, making observations and biometric measurements on: plant height, length and number of shoots, number of leaves on the rosette and on the plant, and the comparative highlighting of the morpho-anatomical and biochemical peculiarities with adaptive characters of the plant organs in the climatic conditions of the country of the cultivated plants and the native spontaneous flora.

The most important solved scientific problem in the thesis consist in the elaboration of the in vitro multiplication technology of the species Lycium barbarum L. and the obtaining of the uncontaminated, uniform and homogeneous quality propagating material for goji varieties in the climatic conditions of the Republic of Moldova. The planting material obtained through in vitro culture will contribute to the establishment of modern plantations in the Republic of Moldova and the organization in GBNI of the collection of fruit bushes.

The theoretical significance. The obtained results allow us to mention that, by using in vitro multiplication, it confirms the increase of the quantity of planting material and the improvement of its quality. Thus, this study highlighted the elucidation of biological features of plant growth and development based on phenological, biometric, anatomical and biochemical aspects in climatic conditions in the Republic of Moldova, data that would contribute to proper training of farmers on the use of drought-resistant and highquality varieties.

The applicative value of the work The scheme of the technology for obtaining healthy and homogeneous planting material for goji varieties for the Republic of Moldova was elaborated. The research results are used in the Embryology and Biotechnology Laboratory of GBNI. The planting material obtained served as a source to initiate the creation of the collection of 5 goji varieties from GBNI: ’Amber Sweet’, ’Erma’, ’Ning Xia N1’, ’New Big’ and ’Licurici’ (variety improved by scientific researchers of the Biotechnology and Embryology Laboratory of GBNI, is currently on the CSTSP test commission). In vitro multiplied goji plants can serve as planting material for the establishment of modern goji plantations in large areas of the Republic of Moldova.

Implementation of scientific results. Based on the scientific research carried out, the in vitro multiplication methods of the Lycium barbarum species were implemented applied in economic contracts made in the Laboratory of Embryology and Biotechnology. The results obtained from phenological and biochemical investigations will enrich the spectrum of the existing berry sector in the Republic of Moldova. At the same time, they represent scientific didactic material for the specialties: Botany, Ecology, Pharmacy and in educational institutions with botanical and agricultural profile, as well as contracts with private beneficiaries, farmers and amateurs interested in goji varieties.