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Molecular Analysis of Different Genotypes of Maize Used in Agriculture


Author: Bondarenco Ecaterina
Degree:doctor of biology
Speciality: 03.00.15 - Genetics
Year:2010
Scientific adviser: Nicolae Barbacar
doctor habilitat, professor, Institute of Genetics, Physiology and Plant Protection, Academy of Sciences of Moldova
Institution: Institute of Genetics and Plant Physiology of the ASM
Scientific council: DH 10-03.00.15
Institute of Genetics and Plant Physiology of the ASM

Status

The thesis was presented on the 22 April, 2010
Approved by NCAA on the 5 July, 2010

Abstract

Adobe PDF document2.00 Mb / in romanian

Keywords

molecular genetics, gene expression, molecular markers, maize (corn), genotypes with valuable traits, stress factors, fungi infection, water scarcity

Summary

The paper consists of: Introduction, 4 chapters, Conclusions and Recommendations, 184 bibliographic sources, a total volume of 118 pages, containing 10 tables and 53 figures. The obtained results have been published in 8 scientific papers.

Research Goal: Expression pattern studies of LTP (Lipid Transfer Protein) and PK (Protein Kinase) genes, presumably involved in plant resistance to biotic and abiotic factors, in maize genotypes with agriculturally valuable traits.

Objectives: to develop specific primers on the basis of the primary structure of transcripts identified in maize anthers, as well as of some members of LTP and PK multigene families and to identify molecular markers eventually associated with valuable traits of maize; to study the expression patterns of the selected LTP and PK genes in maize genotypes grown under stress (fungi infection or water deficiency); 3) to carry out SCAR analysis of agriculturally valuable maize genotypes.

Scientific Innovation: 2 new transcripts of the genes that encode pathogenesis-related proteins and 5 genomic fragments of DNA are identified and molecular characterized. The primary structure of one of the transcripts and its presumable aminoacid sequence was registered in NCBI database (accession numbers GQ507368 and ACX31132, respectively) and can be freely accessed by the international scientific community. For the first time for the Republic of Moldova, a molecular characterization of maize genotypes based on SCAR-analysis is proposed.

Theoretical Significance: The obtained expression profiles of the LTP and PK genes could be adjoined to the already known data and could serve as basis for future research. Practical Importance: The resulted DNA profiles can be used during comparative studies of different maize genotypes, during the estimation of the varieties’ purity.

Implementation of Scientific Results: The primers developed during the research are used in the laboratory of Molecular Genetics of the Institute of Genetics and Plant Phisiology for comparative studies of different maize genotypes.