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Genetic Control Peculiarities of Tomato Resistance to Fusarium Root Rot


Author: Rotaru Ludmila
Degree:doctor of biology
Speciality: 03.00.15 - Genetics
Year:2011
Scientific adviser: Galina Lupaşcu
doctor habilitat, professor, Institute of Genetics, Physiology and Plant Protection of the ASM
Institution: Institute of Genetics, Physiology and Plant Protection of the ASM
Scientific council: DH 10-03.00.15-27.03.08
Institute of Genetics, Physiology and Plant Protection of the ASM

Status

The thesis was presented on the 22 September, 2011
Approved by NCAA on the 11 November, 2011

Abstract

Adobe PDF document – 0.43 Mb / in romanian

Keywords

tomato, Fusarium root rot, resistance, productivity indices, gene actions and interactions, heritability, molecular markers.

Summary

The thesis consists of introduction, 5 chapters, conclusions, practical recommendations, bibliography of 222 sources, a total of 145 pages, including 30 tables and 38 figures. The research results are published in 18 scientific papers.

Study domain: 03.00.15 – Genetics.

Research goal: to determine the genetic control peculiarities of tomato resistance to Fusarium root rot and to reveal the promising forms.

Objectives: to establish the species composition of tomato root rot causals agents, the characteristics of genotype x Fusarium spp. interaction at different temperature conditions and fungi enzymatic activities; to reveal the peculiarities of gene interactions involved in the development of Fusarium root rot resistance and some other valuable traits; to determine the basic genetic parameters of resistance and productivity of tomato; to establish associative links between DNA polymorphism (ISSR markers) and immunological entity of tomato genotypes and populations.

Scientific novelty:for the first time, the following was established: correlations between fungi enzymatic activities, plant-pathogen interactions and resistance to Fusarium spp., the influence of epistasis involved in Fusarium root rot development over gene interactions that control tomato valuable characters, some phenotypic markers of biotic and abiotic stress, the link of the association between the F. oxysporum var. orthoceras filtrate, low temperatures and ISSR-based polymorphisms, which could be used in the immunological screening and identification of genotypes of interest. The important scientific problem solved: was elucidated the genetic factors involved in the reaction of tomato to the fusarium root rot, the interactions tomato x Fusarium spp. x temperature¸ gene interactions, especially – the dublicate epistasis.

Theoretical significance: it was found that the resistance to Fusarium root rot are controlled by the dominant, additive, and dublicate epistasis, influented by the strong interactions genotype x Fusarium x medium, in the wich genotype role are key factor. Practical value: virulent pathogens identification tests have been developed; genotypes/populations of tomato with resistance to Fusarium root rot and valuable production indices have been identified.

Implementation of scientific results: hybrid populations of F3 BC (Costral x Mestnâi) x Mestnâi, F4 (Costral x Mestnâi), F3 BC (Noviciok x Balkan) x Balkan, F4 Sunmark x Mestnâi represent valuable starting material for breeding and are used in the laboratory of Applied Genetics and Center of Vegetal Genetic Resources of IGPP, ASM.