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Conservarea in situ şi ex situ a resurselor genetice forestiere de stejar (Quercus robur) şi gorun (Quercus petraea) din Republica Moldova


Author: Dragoş Postolache
Degree:doctor of biology
Speciality: 03.00.05 - Botany
Year:2007
Scientific adviser: Alexandru Ciubotaru
doctor habilitat, professor, Botanical Garden (Institute) of the ASM
Institution:
Scientific council:

Status

The thesis was presented on the 22 December, 2006
Approved by NCAA on the 1 March, 2007

Abstract

Adobe PDF document0.69 Mb / in romanian

Thesis

CZU 581.632.2:[581.15:581.169](478)

Adobe PDF document 3.55 Mb / in romanian
126 pages


Keywords

forest genetic resources, genetic diversity, in situ conservation, ex situ conservation, pedunculate oak (Quercus robur L.), sessile oak (Quercus petraea (Matt.)Liebl.), molecular genetic markers, haplotype, somatic embryogenesis, cryopreservation

Summary

The present PhD thesis represents a complex study of in situ and ex situ conservation of forest genetic resources of pedunculate oak and sessile oak from the Republic of Moldova because it includes study methods and results from different biological research fields (molecular genetics, phylogeography, embryology, cryobiology, ecology, botanic and phytosociology).

In the framework of this thesis have been identified and described 13 forest genetic resources of pedunculate oak (Quercus robur L.) and 11 forest genetic resources of sessile oak (Quercus petraea). For each forest genetic resources was realized a description based on 36 descriptors and a scheme map. The new described forest genetic resources would be advised to be included in the national protected area fund.

It is recommended the core area of forest genetic resources of pedunculate oak to be at least 19,32ha and for forest genetic resources of sessile oak to be minimum of 12,01ha. The buffer zone of forest genetic resources of pedunculate oak or sessile oak needs to be 800-1000m.

It was realized for the first time in the Republic of Moldova the assessment of genetic diversity in some oak populations by studying the cpDNA polymorphism. The study comprised 15th oaks populations where six haplotypes have been identified (H4, H15, H16, H17, H30, H31), that belong to two cpDNA lineages: lineage A (H4, H30, H31) and lineage E (H15, H16, H17).

The analysis of the cpDNA approach could be of value for conservation efforts by providing a tool for measuring genetic diversity within and between populations.

In the framework of this thesis it was used the static ex situ conservation for the conservation of valuable oaks genotypes.

For the medium and long-term preservation of somatic embryos should be adapted a strategy to encapsulate somatic embryos in advanced cotyledonary stages 4 and 5.

Precotyledonary and early cotyledonary encapsulated somatic embryos of valuable oaks genotypes could be cryopreserved. Although the growth capacity is little, the survived somatic embryos may assure the continuity of embryogenic lines and the cryopreservation method is efficient for long term ex situ conservation of valuable oaks genotypes.