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The biological argumentation of the application of genus Rheum plant’s extracts as a means of plant protection


Author: Gladcaia Alla
Degree:doctor of biology
Speciality: 06.01.11 - Plant Protection
Year:2020
Scientific adviser: Leonid Voloşciuc
doctor habilitat, professor, Institute for Plant Protection and Ecological Agriculture of the ASM
Scientific consultant: Tudor Nastase
doctor, associate professor (docent)
Institution: Institute of Genetics, Physiology and Plant Protection of the ASM

Status

The thesis was presented on the 5 November, 2019
Approved by NCAA on the 28 February, 2020

Abstract

Adobe PDF document1.40 Mb / in romanian
Adobe PDF document1.40 Mb / in russian

Thesis

CZU 635.48:632.937:581.135

Adobe PDF document 4.00 Mb / in russian
156 pages


Keywords

Rheum rhaponticum extracts, biologic activity, Cucurbitaceae, Sphaerotheca fuliginea, Fusarium, Aphididae, Sitotroga cerealella

Summary

Thesis consistsof the introduction, four chapters, main conclusions and recommendations, bibliography of 233 sources, 120 pages of the main text, 24 tables, 33 figures and 5 supplements. The investigation results were published in 20 scientific papers.

Domain of research: Biological sciences. Aim of research: To determine the composition of bioactive substances in R. rhaponticum plants extracts and to evaluate the possibility of using these extracts as plant protection products.

Objectives: Optimization of the genus Rheum plants cultivation; determination of the composition and location of the main rhubarb BAS and the way of extraction; assessment of the effectiveness of rhubarb extracts in the protection of crops against pests and diseases.

Scientific novelty and originality. The localization zones and the composition of the rhubarb BAS have been identified, the optimum method for their extraction has been developed. The types of biological activity of R. rhaponticum extracts on test objects from different taxonomic groups have been determined. Microbiological preparations were used to optimize the technology of the genus Rheum plants growing in the Republic of Moldova.

The important scientific problem solved in the respective domain consists in the scientific substantiation of the R. rhaponticum fungicidal, stimulating, anti-fatigue, insecticidal types of activity, which led to the development of methods for using extracts to stimulate seed germination, to control phytopathogens and pests and allowed to determine their biological effectivenes in plant protection.

The theoretical significance. The main active BAS of Rheum extracts have been identified and the type of their action on phytopathogens and pests of agricultural plants was determined. The practical significance of the work. Methods for obtaining and using of rhubarb extracts to protect agricultural plants from pathogens and pests have been determined; the technology of rhubarb cultivation in the Republic of Moldova conditions have been optimized.

Implementation of the scientific results.The obtained results were used for the biotechnology course elaboration at the State University of the Republic of Moldova . The extracts obtained were tested in the premises GT “Vartic” on greenhouse cucumber “Artist F1” plantation, 2000 m2 square, for environmental protection of powdery mildew.